Journal: Applied and Environmental Microbiology

Article Title: Three alginate lyases provide a new gut Bacteroides ovatus isolate with the ability to grow on alginate

doi: 10.1128/aem.01185-23

Figure Lengend Snippet: Selected bacterial species identified from the IGC to be tested for growth on alginate

Article Snippet: TABLE 1 Species PL families identified based on IGC Strain identifier/DSMZ no Bacteroides acidifaciens PL5, PL6, PL15, PL17, PL32, PL34 A40/DSM 15896 Bacteroides cellulosilyticus PL6, PL15, PL17, PL34 CRE21/DSM 14838 Bacteroides clarus PL6, PL15, PL17, PL34 JCM 16067/DSM 22519 Bacteroides eggerthii PL6, PL15, PL17, PL34 ATCC 27754/DSM 20697 Bacteroides finegoldii PL6, PL15, PL17, PL34 JCM 13345/DSM 17565 Bacteroides ovatus PL5, PL6, PL15, PL17, PL34 ATCC 8483/DSM 1896 Bacteroides stercorirosoris PL6, PL15, PL17, PL34 JCM 17103/DSM 26884 Bacteroides thetaiotaomicron PL6, PL15, PL17, PL34 ATCC 29148/DSM 2079 Bacteroides xylanisolvens PL6, PL15, PL17, PL32, PL34 CCUG 53782/DSM 18836 Open in a separate window Selected bacterial species identified from the IGC to be tested for growth on alginate. . Growth of selected Bacteroides species on alginate The selected bacteria ( ) were cultivated with alginate as a sole carbon source supplemented with the endo -acting alginate lyase A1-I from the marine bacterium Sphingomonas sp. A1 ( 55 , 56 ) producing mainly AOSs of DP2−4 (Fig. S1), to mimic cross-feeding in the HGM.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Three alginate lyases provide a new gut Bacteroides ovatus isolate with the ability to grow on alginate

doi: 10.1128/aem.01185-23

Figure Lengend Snippet: Genes of a PUL in Bacteroides ovatus CP926 and putative functions of the 12 encoded proteins as identified by closest hit using UniProt

Article Snippet: TABLE 1 Species PL families identified based on IGC Strain identifier/DSMZ no Bacteroides acidifaciens PL5, PL6, PL15, PL17, PL32, PL34 A40/DSM 15896 Bacteroides cellulosilyticus PL6, PL15, PL17, PL34 CRE21/DSM 14838 Bacteroides clarus PL6, PL15, PL17, PL34 JCM 16067/DSM 22519 Bacteroides eggerthii PL6, PL15, PL17, PL34 ATCC 27754/DSM 20697 Bacteroides finegoldii PL6, PL15, PL17, PL34 JCM 13345/DSM 17565 Bacteroides ovatus PL5, PL6, PL15, PL17, PL34 ATCC 8483/DSM 1896 Bacteroides stercorirosoris PL6, PL15, PL17, PL34 JCM 17103/DSM 26884 Bacteroides thetaiotaomicron PL6, PL15, PL17, PL34 ATCC 29148/DSM 2079 Bacteroides xylanisolvens PL6, PL15, PL17, PL32, PL34 CCUG 53782/DSM 18836 Open in a separate window Selected bacterial species identified from the IGC to be tested for growth on alginate. . Growth of selected Bacteroides species on alginate The selected bacteria ( ) were cultivated with alginate as a sole carbon source supplemented with the endo -acting alginate lyase A1-I from the marine bacterium Sphingomonas sp. A1 ( 55 , 56 ) producing mainly AOSs of DP2−4 (Fig. S1), to mimic cross-feeding in the HGM.

Techniques: Membrane, Activity Assay

Journal: Applied and Environmental Microbiology

Article Title: Three alginate lyases provide a new gut Bacteroides ovatus isolate with the ability to grow on alginate

doi: 10.1128/aem.01185-23

Figure Lengend Snippet: Kinetic parameters for Bo PL6, Bo PL17, and Bo PL38 a

Article Snippet: TABLE 1 Species PL families identified based on IGC Strain identifier/DSMZ no Bacteroides acidifaciens PL5, PL6, PL15, PL17, PL32, PL34 A40/DSM 15896 Bacteroides cellulosilyticus PL6, PL15, PL17, PL34 CRE21/DSM 14838 Bacteroides clarus PL6, PL15, PL17, PL34 JCM 16067/DSM 22519 Bacteroides eggerthii PL6, PL15, PL17, PL34 ATCC 27754/DSM 20697 Bacteroides finegoldii PL6, PL15, PL17, PL34 JCM 13345/DSM 17565 Bacteroides ovatus PL5, PL6, PL15, PL17, PL34 ATCC 8483/DSM 1896 Bacteroides stercorirosoris PL6, PL15, PL17, PL34 JCM 17103/DSM 26884 Bacteroides thetaiotaomicron PL6, PL15, PL17, PL34 ATCC 29148/DSM 2079 Bacteroides xylanisolvens PL6, PL15, PL17, PL32, PL34 CCUG 53782/DSM 18836 Open in a separate window Selected bacterial species identified from the IGC to be tested for growth on alginate. . Growth of selected Bacteroides species on alginate The selected bacteria ( ) were cultivated with alginate as a sole carbon source supplemented with the endo -acting alginate lyase A1-I from the marine bacterium Sphingomonas sp. A1 ( 55 , 56 ) producing mainly AOSs of DP2−4 (Fig. S1), to mimic cross-feeding in the HGM.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Three alginate lyases provide a new gut Bacteroides ovatus isolate with the ability to grow on alginate

doi: 10.1128/aem.01185-23

Figure Lengend Snippet: Relative abundance (fold change) of PUL-encoded proteins identified in pellet or supernatant from Bacteroides ovatus CP926 grown with alginate as the sole carbon source compared to glucose a

Article Snippet: TABLE 1 Species PL families identified based on IGC Strain identifier/DSMZ no Bacteroides acidifaciens PL5, PL6, PL15, PL17, PL32, PL34 A40/DSM 15896 Bacteroides cellulosilyticus PL6, PL15, PL17, PL34 CRE21/DSM 14838 Bacteroides clarus PL6, PL15, PL17, PL34 JCM 16067/DSM 22519 Bacteroides eggerthii PL6, PL15, PL17, PL34 ATCC 27754/DSM 20697 Bacteroides finegoldii PL6, PL15, PL17, PL34 JCM 13345/DSM 17565 Bacteroides ovatus PL5, PL6, PL15, PL17, PL34 ATCC 8483/DSM 1896 Bacteroides stercorirosoris PL6, PL15, PL17, PL34 JCM 17103/DSM 26884 Bacteroides thetaiotaomicron PL6, PL15, PL17, PL34 ATCC 29148/DSM 2079 Bacteroides xylanisolvens PL6, PL15, PL17, PL32, PL34 CCUG 53782/DSM 18836 Open in a separate window Selected bacterial species identified from the IGC to be tested for growth on alginate. . Growth of selected Bacteroides species on alginate The selected bacteria ( ) were cultivated with alginate as a sole carbon source supplemented with the endo -acting alginate lyase A1-I from the marine bacterium Sphingomonas sp. A1 ( 55 , 56 ) producing mainly AOSs of DP2−4 (Fig. S1), to mimic cross-feeding in the HGM.

Techniques: